The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

The Viewing Head

The viewing head has a 45&deg incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Epi-fluorescence Illumination

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

Fluorescence

Filters

	Filter Set	Excitation Filter	Emission Filter
	B	450-490nm	520nm
	G	510-560nm	590nm

Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band	Peak Wavelength
UV	334nm, 365nm
V	405nm
B	436nm
G	546nm
Y	578nm

Specifications

Optical System

Infinity-corrected

Head

Trinocular, 45° incline

Interpupillary Adjustment

Siedentopf compensation-free gemel, 50mm-75mm

Ocular Diameter

30mm

Ocular Diopters

right ocular

Eyepieces

10X, 25X

Photo-port

height-adjustable 23mm tube

Objective Lenses

Infinity-corrected

Objective Turret

Quadruple

Focusing System

Focus Adjustment	Coaxial Coarse/Fine, adjustable tension
Fine Division	0.002mm

Stage

fixed-height, mechanical X-Y translation

Stage Dimensions

172mm x 242mm

Stage X-Y Travel Range

75mm x 40mm

Specimen Holders

Terasaki plate, Petri dish

Episcopic Illumination (Fluorescence)

100W mercury HBO arc-lamp, centerable

Transmitted Illumination

12V 30W halogen
variable intensity

Condenser

NA 0.3, iris diaphragm, filter slot

Power

110VAC 60Hz

Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness
4X	plan fluor	0.15	28mm	1.5mm
10X	plan fluor, phase-contrast	0.30	3mm	1.5mm
20X	plan fluor, phase-contrast	0.50	4.27mm	1.5mm
40X	plan fluor	0.60	2.23mm	1.5mm

Packing List:

One microscope body

Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X

One pair 10X eyepieces

One pair 25X eyepieces

One phase-contrast annulus board

One top-mount illuminator

One rear-mount fluorescence illuminator

One power supply for fluorescence illuminator

One UV blocking shield

One 30W halogen bulb

One 100W mercury bulb

One dust cover

Color filters

Two power cords

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